1 Introduction

Groundnut is an important food and cash crop in West Africa. One of the largest producers of groundnut is Nigeria. It is either grown for its nut, oil or its vegetative residue (haulms). In northern Nigeria where the crop is mostly grown, Kaduna, Kano, Adamawa, Niger, Bauchi, Bornu and Taraba states, accounted for close to 80% of the total production (Abalu and Harkness 1979).

The crop has several agronomic values which include drought tolerant and ability to produce some yield in soils that are too poor for cultivation of other leguminous crops like common beans (Anchirinah et al. 2001; Azam-Ali et al. 2001). Most of the cultivated areas in Africa are poor sandy soils using high rates of chemical fertilizers. Groundnut provides livelihood to many African farmers and it is known to provide cheap sources of quality protein (22-30%) and oil (42- 52%) on the basis of dry seed analysis (Asibuo et al. 2008), rich source of P, Ca, Mg and P (Savage and Keenan 1994).

P element is an essential macro-nutrient needed for crop growth and high yield. It is mainly supplied through chemical fertilizer application (George and Richardson 2008). P fertilizer is also needed in many plant vital processes such as photosynthesis, root formation and nitrogen

fixation (Grant et al. 2000), energy transformation and seed formation (Asibuo et al. 2008)

Studies have shown that majority of the world soils are deficient in zinc. Yield loss and reduction in the nutritional quality of crops have been accounted for by micronutrient (Zn) deficiency in soils and crops worldwide (Sillanpää 1982). Aboyeji et al. (2019) found that the effect of zinc was not significant on the vegetative parameters, but application of 8 kg Zn·ha^-1 significantly increased number of seeds, weight of seeds, seed yield per hectare, and seed quality. It is estimated that almost half of the soils in the world are deficient in zinc. Zn is known to be one of the eight essential micronutrients needed for plants growth and development.

When the response of plants to one nutrient increases with an increase in the level of the other nutrient, the interaction is said to be positive and the nutrients are said to be synergistic. Conversely, when the response to one nutrient decreases with an increase in the level of the other nutrient, the interaction is said to be negative and the two nutrients are said to be antagonistic (Gomez and Gomez 1984).

P and Zn are both essential nutrients needed for plant growth, but their combined effect at a certain level could be antagonistic especially when the application of soil-P is higher. Higher application of P may cause slower uptake of Zn by the plants or inadequate of Zn in the soil (Mengel and Kirkby 1987), this process results in yield decline in many crops.

Heavy metals have high atomic number and are toxic substances even at low concentrations (Martinez and Tabatabai 1997). These elements are regarded toxic (Wolnik et al. 1993) and classified as carcinogenic (Oymen et al. 2015). The major routes of heavy metal inputs to agricultural soils include atmospheric deposition, sewage sludge, animal manures, agrochemicals and inorganic fertilizers (Nicholson et al. 1998). Losses occur through uptake in crops or livestock products, leaching and soil erosion.

Phosphorus is an important element that interferes with zinc uptake, as zinc uptake by plants reduces by increasing phosphorus in soil. High levels of phosphorus may decrease the availability of zinc or the onset of zinc deficiency associated with phosphorus fertilization may be due to plant physiological factors. One of the causes of the current stagnating yield levels is the deficiency or imbalance of nutrients resulting from nutrient antagonisms (Lobell 2009). Zn and P fertilizers are both essential in crop production but the application of the two fertilizers may have antagonistic and synergistic effects at a particular rate. The study was therefore conducted to determine the levels of interaction of Zn and P fertilizers on the performance, yield, some minerals and heavy metals composition of groundnut grown on an Alfisol of derived guinea savanna.

On the basis of this objective, it was hypothesized that, the interaction of Zn and P fertilizers would have effect on the performance, minerals and heavy metal composition of groundnut. Experiments were therefore carried out to validate this hypothesis; which level of combined or sole applications of Zn and P would improve performance, increase yield and mineral elements without necessarily increasing the heavy metals composition of the test crop grown on an Alfisol.

2 Materials and Methods

3 Results

4 Discussion

Constraints to groundnut production in Nigeria may be due to deficiency of macro and micronutrients and possibly due to nutrients interactions and or poor nutrient retention ability of the soil. One of the most important fertilizer management strategies towards improving crop response to application of fertilizer is to understand or identify synergisms and antagonisms of different types of nutrient elements.

Synergism nutrient interaction is synergistic where the yield due to the combined application of two nutrients is more than the yield expected on the basis of the effects from the individual applications of the nutrients; while antagonism nutrient interaction is antagonistic where the yield due to the combined application of two nutrients is less than the yield expected on the basis of the effects from the individual applications of the nutrients (René et al. 2017). Nutrient imbalance or interaction in crops is probably one of the most important factors affecting yields of annual crops (Fageria et al. 2014).

Application of varying rates of Zn and P on groundnut revealed that the two nutrients have effect on the vegetative growth (plant height, plant spread), yield, yield parameters, nutrient concentration and heavy metals accumulation of groundnut. Application of Zn increased plant height and spread though the effect was not significant on the parameters. Increase in the vegetative parameters may be due to that Zn encourages the vegetative growth and increased plant capacity for building metabolites. It could also be as a result of auxin production by the plants which facilitated production of more plant cells. Similar findings were from Tomar et al. (1990); Saxena and Chandel (1997) where they reported that auxin and better dry matter production are indicators that application of Zn enhanced the plant growth.

P is essentially required for healthy growth with efficient root system and profuse nodulation which in turn can affect the N₂-fixation potential (Kwari 2005). P is considered as a limiting factor in plant nutrition due to the deficiency of available soluble phosphate in the soil (Uma Maheswar and Sathiyavani 2012). Application of P fertilizer at 120 kg P ha^-1 significantly increased plant height and spread though statistically similar with the application of 80 kg P ha^-1. The increase in the vegetative growth of groundnut resulting from the application of P fertilizer could be ascribed to the moderate level of available P in the soil which may not be able to sustain the crop to maturity. It could also be as a result of the beneficial effects of phosphates in the process of photosynthesis, nitrogen fixation and other vital physiological processes. The non- or zero interaction between Zn and P on the vegetative parameters could be that the applied rates for the two nutrients were adequate for the vegetative growth of groundnut.

Deficiency of soil Zn is common in many climatic regions, particularly in sandy soil where it causes yield decrease and low yield quality of groundnut. Zn functions in plants are activity related. Zn activates many plant enzymes such as auxin which assists in producing more cells and dry matter thus yield increase (Devlin and Withan 1983).

Significant increase in yield and yield parameters as a result of application of 8 kg Zn ha^-1 could be ascribed to the improved vegetative growth which facilitated production of a greater number of nodules and in turn increased pod yield as a result of increased rate of photosynthesis and sufficient nutrient availability. These results agree with those of Helpyati (2001) and Sumangala (2003).

The increases in yield and its attributes may be due to the fact that phosphorus is known to help in the development of more extensive root system (Kamara et al. 2011) and thus enables plants to absorb more water and nutrients from the depth of the soil. This in turn could enhance the plant’s ability to produce more assimilates which are reflected in the high biomass production. Application of P, Ca and B fertilizers increased nutrients availability to the crop during the growing season which leads to greater utilization of assimilates into the pods and ultimately increased number of filled pods and shelling percentage (Kamara et al. 2011). In a similar experiment, El-Desouky et al. (1999) found that application of P fertilizer increased number and weight of nodules, N-activity and yield of groundnut.

The result also indicated that zero application of phosphorus fertilizer resulted in the least values for yield and all the yield parameters. This could be ascribed to reduction in biomass, poor root nodulation and development which may affect nitrogen fixation. Kwari (2005) reported that, rhizobia population and legume root development are a function N₂ fixing potential.

Interaction of application of Zn and P fertilizer on yield and yield parameters revealed that there was synergistic effect of the two fertilizers at 8 kg Zn ha^-1 and 80 kg P ha^-1. Antagonistic effect was observed when there was reduction in values for yield and yield parameters at the application of 120 kg P ha^-1. This could be attributed to decrease in Zn uptake which precipitated when P was applied, this reaction will make Zn not to be available to the crops. Zinc can also get precipitated as Zn phosphate on addition of phosphate fertilizers (Lambert et al. 2007). It could also be that at 120 kg P ha^-1 plants have accumulated excess P thereby causing reduced Zn uptake (Salimpour et al. 2010).

Zinc is a cation that interacts with all plant nutrients in the soil or as anions when absorbed by plants. There was a significant interaction between Zn and P on the concentration of P, K, Ca and Mg on groundnut seeds. Results showed that application of Zn and P fertilizers at 8 kg Zn ha^-1 and 80 kg ha^-1 respectively resulted in synergistic effect which made the nutrients elements (P, K, Ca and Mg) available to groundnut.

Plants are capable of absorbing range of necessary nutrient elements from the soil as micronutrients. The soil contains essential heavy metals (Fe, Cu, Mn, Cr, Cd, Zn, As e.t.c) necessary for plant growth and development. Accumulation of heavy metals in plants could result in growth inhibition and toxicity (Hall 2002; Lasat 2002).

Application of Zn fertilizers reduced values for all the heavy metals in groundnut seeds except Zn. Reduction in values of these heavy metals could be attributed to antagonistic effect of Zn. Concentration of Fe increased in plant shoot that are deficient in Zn (Imtiaz et al. 2003). Chaudhry et al. (1973) reported an antagonistic response on rice yield of the Cu x Zn interaction. Zn and Cd compete for uptake and translocation by plants, P-fertilizers contains Zn as contaminants which may reduce accumulation of Cd in crops (Grant 2011).

Superphosphate fertilizers contain in addition to nutrient elements trace metal impurities like Cd, Pb or Hg (Oyedele et al. 2006). Among the contributors of heavy metals into agricultural soils are P-fertilizers. This study revealed that application of 120 kg P ha^-1 increased the concentration of all the heavy metals in groundnut seed. This could be as a result of impurities present in P fertilizer. Phosphate rock on the average is known to contain sizable quantity of As, Cd, Cr, Cu, Pb and Zn in the range of 11, 25, 188, 32, 10, and 239 mg kg respectively (Mortvedt and Beaton 1995). Thomas et al. (2012) in their experiment concluded that, the increase in the concentration of the heavy metals in the soil after the experiment shows that phosphate fertilizers contribute greatly to heavy metal content in soils.

The results revealed that application of Zn and P fertilizers had interaction effects on the availability of heavy metals in the soil and that all the heavy metals detected from the seeds were within the permissible limits as recommended as recommended by (FAO/WHO, 2010).

5 Conclusion

Application of 8 kg Zn ha^-1 and 120 kg P ha^-1 showed synergistic effect on the vegetative parameters and this was similar to the application of 8 kg Zn ha^-1 and 80 kg P ha^-1. The interaction of Zn and P also showed synergistic effect on the yield and yield parameters when 8 kg Zn ha^-1 and 80 kg P ha^-1 was applied and antagonistic effect when 8 kg Zn ha^-1 and 120 kg P ha^-1 was applied. Therefore application 8 kg Zn ha^-1 and 80 kg kg P ha^-1 will be adequate for improved yield, yield parameters and nutrients availability without increasing the heavy metals concentration of groundnut seed grown on an Alfisol.